YRR1 Regulation Network

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Le Crom S. et al.

FIGURES

This section of the related publication web site contains all the figures include in the original article and supplemental data provide for a better understanding on how the results were obtained. All the figures can be accessed througout a table figure index and/or a thumbnail representation of each figure.

Table figure index of all the figures presented in this web site :

Hunting for Yrr1p consensus site :
The Yrr1p global regulation network
- Sumary table for the up-regulated genes found with microarray analyses
- General diagram including YRR1 to the PDR network

Thumbnail representation of all the figures gathered in this web site :

Experimental strategy :
Zn2Cys6 zinc finger transcription factor family phylogentic tree	Schematical construction strategy used to search for Yrr1p regulated genes
Physiological properties :
Verification of correct Yrr1*GAD chimera production by northern blot	Growth rate in rich galactose medium for wild-type and Yrr1S*GAD strains	Drug resistance phenotype analysis for physiological properties verification
Microarray analysis :
PCA analysis of Yrr1S*GAD regulated genes after 4 hours of galactose induction	Global analysis of total Yrr1p experiments using PCA and hierarchical clustering
Time course analysis of Yrr1p regulated genes :
Mean time course profile of Yrr1p regulated genes	Classification in main groups of Yrr1p regulated genes using northern blot quantification	Time course of Yrr1p regulated genes observed by northern blot
Hunting for Yrr1p consensus site :
Strategy for the selection of putative binding site on promoter region	EMSA analysis for Yrr1p regulated genes found after microarray experiments	Experimental approach for delinate the AZR1 and SNG1 DNA binding sequence
Complete EMSA study of the AZR1 and SNG1 promoter reduction	Restriction enzyme digestion of the AZR1 and SNG1 200 base pair selected promoter region	Recapitulative table of the data obtained after the previous EMSA promoter reduction
Conclusions and perspectives :
Recapitulative table of up-regulated-genes	The Yrr1p consensus binding sequence	The Yrr1p regulation network