Vent diagram from the comparison of the two experiments describe above.
Looking for co-regulated genes
Another useful way to mine DNA microarray information is to compare transcription profiles between a set of experiments. This is the goal of the "search by transcription profile" link in yMGV homepage. Using this option, we compared the effect of zinc starvation (Lyons et al., 2000) to a deletion of the YFH1 gene encoding a mitochondrial protein responsible for iron binding and storage (Foury et al., 2001). We found 17 genes that are up regulated by more than 3 fold in both experiments.
Vent diagram from the comparison of the two experiments describe above.
| ORF | Name | SGD Function | Link |
| YBR054W | YR02 | Homolog to HSP30 heat shock protein Yro1p | SGD |
| YCR021C | HSP30 | Protein induced by heat shock, ethanol treatment, and entry into stationary phase; located in plasma membrane | SGD |
| YDR343C | HXT6 | hexose transporter | SGD |
| YDR345C | HXT3 | low affinity glucose transporter | SGD |
| YDR534C | FIT1 | Cell wall protein involved in iron uptake | SGD |
| YEL065W | SIT1 | Siderophore Iron Transport | SGD |
| YER145C | FTR1 | Plasma membrane iron permease | SGD |
| YHL040C | ARN1 | Siderophore transporter for triacetylfusarinine C | SGD |
| YHL047C | TAF1 | Zinc finger containing homolog of mammalian TIS11, glucose repressible gene | SGD |
| YLR136C | TIS11 | Zinc finger containing homolog of mammalian TIS11, glucose repressible gene | SGD |
| YMR058W | FET3 | multicopper oxidase | SGD |
| YNL160W | YGP1 | gp37, a glycoprotein synthesized in response to nutrient limitation which is homologous to the sporulation-specific SPS100 gene | SGD |
| YOL158C | ENB1 | Siderophore transporter for enterobactin; AFT1 regulon | SGD |
| YOR382W | FIT2 | Cell wall protein involved in iron transport | SGD |
| YOR383C | FIT3 | Cell wall protein involved in iron transport | SGD |
| YPR145W | ASN1 | asparagine synthetase | SGD |
| YPR160W | GPH1 | glycogen phosphorylase | SGD |
This reveals a common response to zinc and iron starvation in yeast that includes stress proteins (Yro2p, Hsp30p, Ygp1p, Gph1p), metal transporters (Sit1p, Ftr1p, Arn1p, Fet3p, Enb1p) together with unknown genes that were thought to be specifically involved in iron uptake (Ydr534p, Yor382p, Yor383p, Tis11p). This suggests cross-talks between iron and zinc homeostasis.