GENOME-WIDE STUDIES ON THE NUCLEAR PDR3-CONTROLLED RESPONSE TO MITOCHONDRIAL DYSFUNCTION IN YEAST
Frédéric DEVAUX, Elvira CARVAJAL, Scott MOYE-ROWLEY and Claude JACQ
FEBS letters, 2002
ABSTRACT
Gain-of-function mutations in the transcription factors Pdr1p and Pdr3p lead to the up-regulation of genes controlling the plasma membrane properties. Pdr3p is involved in a retrograde response in which mitochondrial dysfunctions activate PDR5, a gene encoding an ABC membrane transporter. We carried out genome-wide analyses of the PDR3-controlled genes activated by the deletion of the mitochondrial DNA. We present evidence showing that PDR1 does not interfere with this PDR3 response. We also showed that the mitochondrially-activated PDR3 response is highly sensitive to both yeast strain variations and carbon sources. These observations explain the apparent discrepancies in published studies and better describe the connections between the mitochondrial state and plasma membrane properties.
 
DATA
Figure 1 genes activated by Pdr3p* in rho0 cells
Figure 2 Effect of PDR1 and the gain-of-function allele, PDR3-7, on the genes activated by Pdr3p* in rho0 cells.
Figure 3 Northern blot analyses of the amount of PDR3 and PDR5 mRNA in rho0 and rho+ cells from different backgrounds grown on different carbon sources.
SUPPLEMENTAL DATA
Table 1 Ratio values from the clusters presented in figure 1 and figure 2.
* PDR3p activated by the mitochondrial dysfunction

For any biological question please contact: Devaux@biologie.ens.fr and Jacq@biologie.ens.fr
 
 
 

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