 figure
1: Procedure for isolating and identifying mRNAs associated with mitochondrion-bound
polysomes.
 mito-polysome
purification protocol[pdf file 66ko]
fractionation
test: ATP2 & COX6 northern blots
see also microarray
images from download page |
 |
| figure
2: Relationship between MLR and localisation of proteins.
biochemical
properties of the orf vs MLR |
 |
| figure
3: : mRNAs encoding mitochondrial proteins found in the two extreme cytosolic
and mitochondrion-bound fractions.
Diehn's paper
homepage(medline:
Nat Genet 2000 May;25(1):58-62)
listing
of the 467 ORFs having MLR > 80 |
 |
| figure
4: 3'UTR of mRNAs with highest MLR can target a gRNA to the vicinity of
mitochondria.
green
RNA principle schema |
 |
| figure
5: The dual origin of the mitochondrial proteome matches the dual location
of mRNAs.
figure
5 with all Karlberg's paper sub-categories
Karlberg's paper
homepage(medline:Yeast
2000 Sep 30;17(3):170-187)
Find
out the predicted origin of a mitochondrial ORF |
 |
| figure 6:
The asymmetric localisation of mRNAs is connected with biochemical functions.
other
pathways implicating mitochondrion localized mRNA |
 |
Last updated november 21th 2001 by MARC
Philippe